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nadA [2018-01-03 16:07:11]
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nadA [2018-01-03 16:07:11]

quinolinate synthetase
locus
BSU27850
pI
5.72
mw
41.33 kDa
protein length
368 aa Sequence Blast
gene length
1104 bp Sequence Blast
function
NAD biosynthesis
product
quinolinate synthetase
essential
no
ec
2.5.1.72
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]

    • Gene

    Coordinates
    2,845,955 → 2,847,061

    The protein

    Catalyzed reaction/ biological activity

  • Glycerone phosphate iminosuccinate = pyridine-2,3-dicarboxylate 2 H2O phosphate (according to Swiss-Prot)

    • Protein family

  • Type 3 subfamily (according to Swiss-Prot)

    • [SW|Cofactors]

  • [4 Fe- 4 S] cluster [Pubmed|18959769]

    • Structure

  • [PDB|4HHE] (from Pyrococcus furiosus, 34% identity) [pubmed|23999292]

    • Expression and Regulation

    Operons

    genes
    [gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]-[gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]-[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]
    description
    [Pubmed|8444804]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8444804], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|510DE075EA964D22C42DEB171509F6CDA600A402|NadR]: repression, [Pubmed|16199587], in [regulon|510DE075EA964D22C42DEB171509F6CDA600A402|NadR regulon]

    • regulation

  • repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|16199587]

    • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|nadB]' [PubMed|20525796]
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    Biological materials

    Mutant

  • BKE27850 (Δ[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATTCGATTGTTTGA, downstream forward: _UP4_TAGATCAGAAATATTTTCCC
  • BKK27850 (Δ[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATTCGATTGTTTGA, downstream forward: _UP4_TAGATCAGAAATATTTTCCC

    • Labs working on this gene/protein

  • [SW|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]

    • References

  • 18959769,16199587,8444804,23999292